The spreading of Alfalfa mosaic virus in lavandin in Croatia
نویسندگان
چکیده
A survey was conducted in 2012 and 2013 to detect the presence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. AMV was detected serologically in 62 samples collected at three different localities, and none of the samples tested positive for CMV. For further analyses, six selected samples of naturally infected lavandin plants originating from different localities were mechanically transmitted to test plants: Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana and Ocimum basilicum, confirming the infectious nature of the disease. Molecular detection was performed by amplification of a 751 bp fragment in all tested samples, using the specific primers CP AMV1/CP AMV2 that amplify the part of the coat protein (CP) gene and 3’-UTR. The RT-PCR products derived from the isolates 371-13 and 373-13 were sequenced (KJ504107 and KJ504108, respectively) and compared with the AMV sequences available in GenBank. CP sequence analysis, conducted using the MEGA5 software, revealed that the isolate 371-13 had the highest nucleotide identity of 99.5% (100% amino acid identity) with an isolate from Argentina originating from Medicago sativa (KC881010), while the sequence of isolate 373-13 had the highest identity with an Italian AMV isolate from Lavandula stoechas (FN667967) of 98.6% (99% amino acid identity). Phylogenetic analysis revealed the clustering of selected isolates into four molecular groups and the lavandin AMV isolates from Croatia grouped into two distinct groups, implying a significant variability within the AMV lavandin population.
منابع مشابه
بررسی ویروس موزاییک یونجه در مناطق شمالی و مرکزی استان خراسان
The study of dispersion of alfalfa mosaic virus (ALMV) infection based on DAS-ELISA indicated that the fields of Alfalfa, potatoes and tomatoes from Chenaran, Ghochan, Shirvan, Mashhad, Neishaboor and Torbat Heydarieh were infected with the virus. The Statistical analysis indicated that the amount of infection did not differ in the surveyed regions and total mean of infection was 53 percent. Th...
متن کاملبررسی ویروس موزاییک یونجه در مناطق شمالی و مرکزی استان خراسان
The study of dispersion of alfalfa mosaic virus (ALMV) infection based on DAS-ELISA indicated that the fields of Alfalfa, potatoes and tomatoes from Chenaran, Ghochan, Shirvan, Mashhad, Neishaboor and Torbat Heydarieh were infected with the virus. The Statistical analysis indicated that the amount of infection did not differ in the surveyed regions and total mean of infection was 53 percent. Th...
متن کاملPartial Epitope Mapping of Alfalfa mosaic Virus and the Effect of Coat Protein Gene Mutation on Aphid Transmission
متن کامل
Incidence, Spatial Patterns, and Associations Among Viruses in Snap Bean and Alfalfa in New York
Beginning in 2000, snap bean (Phaseolus vulgaris L.) crops exhibiting virus-like symptoms on the foliage (leaf distortion, yellow and green mottling, and mosaic) were increasing in frequency on a regional scale across the northern United States and Canada (10,20). These crops also produced fewer pods or pods that were either twisted or necrotic (hence unmarketable). Across the Midwest and north...
متن کاملCharacterization of Cucumber Mosaic Virus From Mint in Tehran Province
Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...
متن کامل